Synaptek-DLS https://synapteklabs.com/ Deep Learning Science Thu, 21 Nov 2024 23:13:28 +0000 en-GB hourly 1 https://wordpress.org/?v=6.7.1 https://synapteklabs.com/wp-content/uploads/2021/04/fav2-50x50.png Synaptek-DLS https://synapteklabs.com/ 32 32 PrP^Sc Formation And Detection Using RT-QuIC https://synapteklabs.com/prpsc-formation-and-detection-using-rt-quic/ Thu, 21 Nov 2024 23:12:03 +0000 https://synapteklabs.com/?p=4845 PrP^Sc Formation & Detection RT-QuIC, which stands for Real-Time Quaking-Induced Conversion, is a sophisticated assay for detecting prions, proteins linked...

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PrP^Sc Formation & Detection

RT-QuIC, which stands for Real-Time Quaking-Induced Conversion, is a sophisticated assay for detecting prions, proteins linked to diseases such as mad cow disease, scrapie, and chronic wasting disease. Here’s how it functions:

Fundamental Concept: It utilizes the prion’s ability to transform normal prion protein (PrP^C) into the infectious PrP^Sc.

Methodology:

  • Substrate: A solution containing recombinant prion protein (rPrP) is used.
  • Seeding: Any prions present in a sample will convert the substrate into amyloid fibrils.
  • Shaking: The mixture is agitated to break apart and multiply the fibrils, accelerating the reaction.

Fluorescence Detection: Changes in fluorescence from dyes binding to fibrils are monitored in real-time.

Advantages:

  • Sensitivity: Detects prions at concentrations that are too low for other detection methods.
  • Specificity: Highly accurate, especially in identifying diseases like sporadic Creutzfeldt-Jakob disease.
  • Speed: Provides quick results compared to traditional bioassays.
  • Flexibility: Can be used with various biological samples like brain tissue, cerebrospinal fluid, and others.

Applications:

  • Diagnosis: Crucial for diagnosing prion diseases before death.
  • Research: Assists in the study of prion strain characteristics, transmission, and potential interventions.

An Essential Tool

RT-QuIC has become an essential tool in the field of prion disease research and diagnostics, providing a rapid, sensitive, and specific method for prion detection.

When considering the detection of PrP^Sc after exposure to the S1 subunit and neutrophil elastase, the RT-QuIC (Real-Time Quaking-Induced Conversion) method can be employed as follows:
  1. PrP^Sc Formation:
    • PrP^Sc arises from the misfolding of the normal prion protein (PrP^C), leading to a form that’s resistant to protease digestion and associated with diseases like scrapie.
  2. Interaction with S1 Subunit:
    • While there’s no direct evidence tying the S1 subunit (from viruses like SARS-CoV-2) to prion conversion, the setup could investigate whether this interaction might influence the conversion or stability of prion proteins.
  3. Role of Neutrophil Elastase (NE):
    • NE, a protease from neutrophils, is known for its protein degradation capabilities:
      • Cleavage: NE might degrade PrP^Sc, potentially affecting detection if not accounted for.
      • Influence on Conversion: NE could theoretically impact prion propagation or conversion, possibly by cleaving PrP^C at sites that promote aggregation.
  4. Detection with RT-QuIC:
    • RT-QuIC Procedure:
      • This assay involves a recombinant PrP substrate in a reaction mix. When PrP^Sc is present, it catalyzes the conversion of the substrate into amyloid fibrils, which can be monitored in real-time by the increase in fluorescence due to the binding of thioflavin T (ThT).
      • Sensitivity: RT-QuIC is highly sensitive to small amounts of PrP^Sc, making it ideal for detecting prions even after potential degradation or modification by NE.
      • Control Reactions: To understand the effects of NE or S1 subunit:
        • Controls would include untreated samples, samples with only NE, samples with only the S1 subunit, and samples with both.
        • If NE or the S1 subunit affects prion detection, the fluorescence kinetics in these conditions would differ from controls.
  5. Experimental Considerations:
    • Inhibition: Using specific NE inhibitors could help clarify if changes in RT-QuIC results are due to NE activity.
    • Viral Protein Interaction: The influence of the S1 subunit on prion dynamics could be explored by comparing the kinetics with and without its presence.
  6. Theoretical Implications:
    • Investigating the S1 subunit’s interaction with prions through RT-QuIC could provide insights into whether viral proteins can modulate prion disease progression or detection.

Why RT-QuIC

RT-QuIC’s ability to detect minute quantities of prions makes it a potent tool for this kind of research, offering a window into how external proteins or enzymes might influence prion detection or disease pathogenesis. However, any findings would require rigorous validation and further study to confirm the implications for prion biology.


 

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Informed Consent is Dead by DocKek https://synapteklabs.com/informed-consent-is-dead-by-dockek/ https://synapteklabs.com/informed-consent-is-dead-by-dockek/#respond Fri, 16 Dec 2022 18:43:38 +0000 https://synapteklabs.com/?p=4633 Why Compulsory Reporting of a Biologic Toxin is the Only Way Is it possible to release a medical product without...

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Why Compulsory Reporting of a Biologic Toxin is the Only Way

Is it possible to release a medical product without providing informed consent to the one receiving it? Like you, a year ago I would have replied with a resounding NO! However, after having been engaged in litigation against a state agency to provide informed consent, the surprising answer is apparently, yes… After suing the Virginia Department of Health under state informed consent statutes it appears impossible to establish legal…

Regardless of this clear error in judgement, there is a post-mortem that must be done in order to determine how best to move forward on a strategic level, aside from the tactical option of filing a notice to appeal before the new year; which is completely dependent on funds, or some other legal organization offering their assistance to continue.

Section 564, Informed Consent & MCM’s

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Introducing Lab Rat #5 https://synapteklabs.com/introducing-lab-rat-5-craig-l-mcgrain/ https://synapteklabs.com/introducing-lab-rat-5-craig-l-mcgrain/#respond Sat, 03 Dec 2022 16:23:16 +0000 https://synapteklabs.com/?p=4623 Doctor Kevin W. McCairn Ph.D. received the new that another great supporter has generously sponsored a lab rat

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Introducing Lab Rat #5 – Craig L. McGrain

ab Rat 5 - Craig L. McGrain

Today, December 3rd 2022, Doctor Kevin W. McCairn Ph.D. received the new that another great supporter has generously sponsored a lab rat ( lab rat #5) to be used in the upcoming rodent Covid Studies. Dr. McCain will be conducting studies to determine the overall affects of the introduction of various Covid-19 vaccines into the brains of the rodents.  Please continue to follow the story of the upcoming experiments here at Synaptek. As the rodents sponsor get to name it, this rat is called Craig L. McGrain.

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Covid19 – The Spartacus Letter Pt.1 https://synapteklabs.com/covid19-the-spartacus-letter-pt-1/ https://synapteklabs.com/covid19-the-spartacus-letter-pt-1/#respond Mon, 28 Nov 2022 02:31:11 +0000 https://synapteklabs.com/?p=4604 Covid19 – The Spartacus Letter Pt.1 Hello, My name is Spartacus, and I’ve had enough. We have been forced to...

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Covid19 – The Spartacus Letter Pt.1

Covid19 – The Spartacus Letter Part 1

Hello, My name is Spartacus, and I’ve had enough.

We have been forced to watch America and the Free World spin into inexorable decline due to a bio-warfare attack.

We, along with countless others, have been victimized and gaslit by propaganda and psychological warfare operations being conducted by an unelected, unaccountable Elite against the American people and our allies.

Our mental and physical health have suffered immensely over the course of the past year and a half.

We have felt the sting of isolation, lock-down, masking, quarantines, and other completely nonsensical acts of healthcare theater that have done absolutely nothing to protect the health or well-being of the public from the ongoing COVID-19 pandemic.

Now, we are watching the medical establishment inject literal poison into millions of our fellow Americans without so much as a fight.

We have been told that we will be fired and denied our livelihoods if we refuse to vaccinate. This was the last straw.

We have spent thousands of hours analyzing leaked footage from Wuhan, scientific papers from primary sources, as well as the paper trails left by the medical establishment.

What we have discovered would shock anyone to their core.

First, we will summarize our findings, and then, we will explain them in detail. References will be placed at the end.

Summary:

• COVID-19 is a blood and blood vessel disease. SARS-CoV-2 infects the lining of human blood vessels, causing them to leak into the lungs.

• Current treatment protocols (e.g. invasive ventilation) are actively harmful to patients, accelerating oxidative stress and causing severe VILI (ventilator-induced lung injuries). The continued use of ventilators in the absence of any proven medical benefit constitutes mass murder.

• Existing countermeasures are inadequate to slow the spread of what is an aerosolized and potentially wastewater-borne virus, and constitute a form of medical theater.

• Various non-vaccine interventions have been suppressed by both the media and the medical establishment in favor of vaccines and expensive patented drugs.

• The authorities have denied the usefulness of natural immunity against COVID-19, despite the fact that natural immunity confers protection against all of the virus’s proteins, and not just one.

• Vaccines will do more harm than good. The antigen that these vaccines are based on, SARS-CoV- 2 Spike, is a toxic protein. SARS-CoV-2 may have ADE, or antibody-dependent enhancement; current antibodies may not neutralize future strains, but instead help them infect immune cells. Also, vaccinating during a pandemic with a leaky vaccine removes the evolutionary pressure for a virus to become less lethal.

• There is a vast and appalling criminal conspiracy that directly links both Anthony Fauci and Moderna to the Wuhan Institute of Virology.

• COVID-19 vaccine researchers are directly linked to scientists involved in brain-computer interface (“neural lace”) tech, one of whom was indicted for taking grant money from China.

• Independent researchers have discovered mysterious nano-particles inside the vaccines that are not supposed to be present.• The entire pandemic is being used as an excuse for a vast political and economic transformation of ”Western Society” that will enrich the already rich and turn the rest of us into serfs and untouchables.


End of Part 1 – Coming soon, Part 2

Spartacast Episode 4 – https://substack.com/saved/post/70472675

Hey guys, Spartacus, here for a fourth Spartacast.

I’ve spent the past few weeks working on various projects while growing increasingly enraged at the reports I’ve seen. The vaccine-injured are growing in number. They are being given very little consideration by public officials…

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Cosmic Radiation and The ”Spike” – Poison??? https://synapteklabs.com/cosmic-radiation-and-the-spike-poison/ https://synapteklabs.com/cosmic-radiation-and-the-spike-poison/#respond Tue, 25 Oct 2022 17:02:12 +0000 https://synapteklabs.com/?p=4539 The 2P Spike Protein – Poison or Safe? By Guest Author – Spartacus One of the biggest problems I have...

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The 2P Spike Protein – Poison or Safe?

By Guest Author – Spartacus

One of the biggest problems I have with these vaccines is that they’re claiming that 2P Spike is safe for cells to produce simply because it has those proline substitutions that lock it in the prefusion conformation.

Of course, the  CDC has stated that the spike protein of SARS-CoV-2 is harmless, which assures that current Covid-19 vaccines would be safe.

I’ve gotten into shouting matches on Twitter.

I asked people what they thought the final fate of the protein was, and they couldn’t come up with a straight answer.

None, zip, nada! They thought that the 2P Spike was completely inert and immune to any kind of proteolytic cleavage.

Proteolysis is when enzymes cut a protein up like a pair of scissors.

Proteolysis Defined

Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. 

Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein.

It is also important in the regulation of some physiological and cellular processes including apoptosis, as well as preventing the accumulation of unwanted or misfolded proteins in cells.

Consequently, abnormality in the regulation of proteolysis can cause disease. – https://en.wikipedia.org/wiki/Proteolysis

We know that when Spike is exposed to neutrophil elastase, which is an enzyme commonly released by neutrophils as an inflammatory reaction, it breaks up into small peptide fragments that are each, individually, amyloidogenic.

Alzheimer’s

Amyloid plaques are what you see in Alzheimer’s. Please see following paper – https://pubs.acs.org/doi/10.1021/jacs.2c03925

COVID-19 involves iron dysregulation, oxidative stress, and amyloidosis, and it’s because of the virus’s highly toxic proteins.

That combination is basically an inducer of aging and aging-related disease.

Cosmic Radiation

It’s exactly like an astronaut being exposed to cosmic radiation, biologically speaking.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861125/

https://www.sciencedirect.com/science/article/pii/S0753332221000135

https://cardiab.biomedcentral.com/articles/10.1186/s12933-022-01623-4

https://www.nature.com/articles/s41467-022-29801-8

Combination of Effects

The vax might not induce that precise effect; that inflammatory profile is seen in COVID-19, and it’s very likely due to a combination of effects from the virus. The Spike has some more specific negative effects, particularly endothelial toxicity.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538996/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547916/

SARS-CoV-2

 

WHY???

The real question here is why many of these same tests have not been performed with 2P Spike and cell cultures to see if it shares the same negative effects as the Spike from the virus.

There’s just a total absence of that sort of research.

They’re basically assuming, based on nothing, that it is safe and inert.


About The Author: Spartacus

Spartacus is known to us as the main contributor at The ICENI Bulletins.

The ICENI believe that crimes of unimaginable proportions have taken place, upon the good people of earth.

Also that these crimes are still ongoing, involving high-level public officials, intelligence agencies, and their pharma pawns.

The investigation continues. The perpetrators of this criminal conspiracy are not above the law, nor are they beyond the reach of public scrutiny. – Spartacus

For more information, please visit – https://iceni.substack.com/.

To listen to the podcasts from Spartacus, please visit – https://iceni.substack.com/podcast

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Biologic Toxicity of the Spike Protein? https://synapteklabs.com/biologic-toxicity-of-the-spike-protein/ https://synapteklabs.com/biologic-toxicity-of-the-spike-protein/#respond Sat, 22 Oct 2022 00:04:35 +0000 https://synapteklabs.com/?p=4523 Pena v VDH Lawsuit & How to Follow How do you ask for Informed Consent on the Biologic Toxicity of...

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Pena v VDH Lawsuit & How to Follow

How do you ask for Informed Consent on the Biologic Toxicity of the Spike Protein?

Guest Author: DocKek

  1. When I win I will have established “legal standing” for further suit.
  1. When the declaratory judgement is granted it will be the first instance in the nation where the spike protein were to be legally defined as a biologic toxin and everything making it in the body a toxic substance;
  1. with further notice being mandated to anyone receiving the inoculation of the possibility of a “health-care associated infection” related to long covid.
  1. game. set. match.

Legal Basis

This is meant to be a background on the legal basis for bringing suit for Informed Consent against any that would deny it.

In Virginia. Informed Consent is defined as “providing policies allowing knowing and voluntary agreement, without undue inducement or ANY ELEMENT of force, fraud, deceit, duress, or other form of constraint or coercion, of a person who is capable of exercising free power of choice.”

Shall Include

The “basic elements of information necessary to such consent” (Basic Elements) shall include:

1. A reasonable and comprehensible explanation to the person of the proposed procedures or protocols to be followed, their purposes, including descriptions of any attendant discomforts, and risks and benefits reasonably to be expected;

2. A disclosure of any appropriate alternative procedures or therapies that might be advantageous for the person;

3. An instruction that the person may withdraw their consent and discontinue participation in the human research at any time without prejudice to them;

4. An explanation of any costs or compensation which may accrue to the person and, if applicable, the availability of third party reimbursement for the proposed procedures or protocols; and

5. An offer to answer and answers to any inquiries by the person concerning the procedures and protocols.

Requirements

Every State has defined Informed Consent’s requirements in their own statutes, or state laws.

Informed Consent is required for every medical procedure including Emergency Use Authorisation  treatments.

Meaning each element would necessarily apply to being injected with experimental medical technology.

In order to qualify for a lawsuit a person must first demonstrate themselves as an “aggrieved party” in a “justiciable controversy” developed upon a certain set of circumstances, facts, or reality.

Assertion and Denial of Right

In cases for a Declaratory Judgement the decision will rest upon an assertion and denial of right.

Meaning, in order to sue under a declaratory judgement someone must assert their right to Informed Consent and then have it denied by someone legally compelled/required to act, or perform a statutory duty, like “an offer to answer and answers to any inquiries by the person concerning the procedures and protocols.”

Declaratory judgements are NOT advisory opinions. Meaning they CANNOT be issued over disputed facts.

Issues of Dispute

In cases involving issues of dispute, such as the biologic toxicity of the spike protein, a declaratory judgement will NOT be the correct suit to file, which is why it is important to assert your understanding and receive feedback FIRST, in order to establish a given set of facts to base a lawsuit on.

In my case I was able to inform those required to inform me, and gain an admission that the spike protein for COVID-19 is closely related to the cause of Long COVID.

Long Covid is Recognized

Long COVID is recognized by the US Dept. of Health and Human Services as a disability under the Americans with Disabilities Act. Using these legal definitions, it was a matter of law to see how they matched the legal definition for a toxin, or biologic agent.

Long Covid, being a direct sequelae of a virus of public health threat affords it additional reporting requirements under the law as well.

With the spike protein for COVID-19 being the causative agent of Long COVID, and Long COVID being the direct sequelae for COVID-19, it is a serious medical question as to why it is also used as the therapeutic target for all vaccines.

Everyone!

Everyone has the ability to request Informed Consent from any medical professional or government agency requiring them to do so.

Find out the Informed Consent laws in your state.

Address either government agencies, pharmacies, or administering health professionals if they are familiar with their statutory duty to provide Informed Consent.

Any entity issuing mandates for human research, medical procedures, or health related matters must first provide informed consent by statute. If those mandating are not able to, or have not yet provided Informed Consent, then by what authority can anyone comply?

Ask questions incessantly and you will find the answers as well as how to achieve the result you want.

Case-law, Processes, and Procedures

In every state, in most public libraries is the book judges use to determine cases; In Virginia it is called The Virginia Code, in North Carolina it is The North Carolina General Statutes. Each state has their own case-law, processes, and procedures.

The book will contain the case-law on how statutes have been applied in the past; meaning, how Judges would be compelled to rule today.

Beyond One Man

If you fail the first time, the answer to how to follow-up is usually within the opinion the judge provides for the case.

Read, learn, adjust, and you will eventually succeed.

This is beyond one man, but one man can make a difference, and eventually, if enough know how to follow, men and women will collectively make a difference.

~Many Blessings “DocKek”


About The Author

DocKek’s Daily Dive is a reader-supported publication.

To receive new posts and support his great work, consider becoming a free or paid subscriber.

To Subscribe, please visit – https://dockek.substack.com/

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Rat #2 & 3 Reggie Rat and Roland Rat https://synapteklabs.com/rat-2-3-reggie-rat-and-roland-rat/ https://synapteklabs.com/rat-2-3-reggie-rat-and-roland-rat/#respond Sun, 16 Oct 2022 21:47:26 +0000 https://synapteklabs.com/?p=4437 Welcome Reggie and Roland the #2 & #3 Rats We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series Introducing the next two...

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Welcome Reggie and Roland the #2 & #3 Rats

We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series

Introducing the next two rodent volunteers, #2 & #3 , Reggie Rat and Roland Rat.

The second and third addition to our our very first We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series,

Along with our first , Miracle the Rat, Reggie Rat and Roland Rat are being sponsored by our very good friends from our fanatic community of site supporters here on the WTYL platform, the only live science Covid Research Studies, publicly funded for the public good.

You will see it all live, with all access and as they happen.

Safe and Effective?

You will watch Dr. Kevin McCairn conduct his first of many experiments concerning the harmful effects being reported worldwide from both the SARS-CoV-2 virus, and the gene transfection technologies “vaccines”, which we all have been subjected to. Subjected too, with many, many deaths and injuries being documented on a daily basis.

Some of us were forced to take the shots as a safe and effective option.

For too many, the only option! Harm soon followed for them.

The #1 Cause of Death

Unknown causes of death are vastly becoming overwhelming in many areas.

In Alberta Canada is has been reported to be the number 1 cause of death for certain individuals in 2022.

The actual death certificates state – Cause of Death: Unknown

The Light of Real Science

We find this unconscionable! We aim to bring the light of real science to the dark world of media and politically controlled science.

All of which is falling apart bit by bit. Every day we hear more bad news as the world crumbles before our eyes across the planet.

The main reason rats are used in experiments is because they are similar to humans in many ways. They share a staggering 90% of genes with humans and their bodily systems function similarly to humans. Additionally, rats can be changed genetically which helps researchers study how certain genes can cause specific diseases in humans. – ScienceABC

Join With Us In This Fight

Please consider joining  with us as a We Talk You Listen’s Buy-a-Rat/Name-a-Rat Series Sponsor.

Each lab rat will be handled and treated within our ethical and moral  standard which we hold all science to. All science needs to be help out for the public to see and understand.

Without opinions or conjecture.

They told us to ”Trust The Science’‘, so we aim to do just that.

However, it will be us conducting the experiments and collecting the data. We also intend to publish the outcomes and finding as soon a possible.

We do all this for the ”Public Good”. Please help us and sponsor a rat!

We rely on all our fanatic support we receive, to bring you the bets of what science can bring.

Thanks you so much for being here with us!

Best Regards.

WTYL Team.


If you would like to join with us and Sponsor a Rat in the WTYL Rat Studies please click the following link –  https://synapteklabs.com/product/wtyl-rodent-adoption/

WTYL Rodent Adoption “Buy a Rat, Name a Rat”

 

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Sponsor-a-Rat Name-a-Rat – Miracle The Rat https://synapteklabs.com/sponsor-a-rat-name-a-rat-introducing-miracle-the-rat/ https://synapteklabs.com/sponsor-a-rat-name-a-rat-introducing-miracle-the-rat/#respond Sun, 09 Oct 2022 02:45:19 +0000 https://synapteklabs.com/?p=4355 Introducing Miracle the Rat We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series Introducing our very first We Talk You Listen’s Sponsor-a-Rat...

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Introducing Miracle the Rat

Miracle the Rat

We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series

Introducing our very first We Talk You Listen’s Sponsor-a-Rat Name-a-Rat Series, Miracle the Rat.

Miracle the Rat is being sponsored by our very good friend Jana from our fanatic community of site supporters here on the WTYL platform, the only live science Covid Research Studies, publicly funded for the public good.

You will see it all live, with all access and as they happen.

Safe and Effective?

You will watch Dr. Kevin McCairn conduct his first of many experiments concerning the harmful effects being reported worldwide from both the SARS-CoV-2 virus, and the gene transfection technologies “vaccines”, which we all have been subjected to. Subjected too, with many, many deaths and injuries being documented on a daily basis.

Some of us were forced to take the shots as a safe and effective option.

For too many, the only option! Harm soon followed for them.

The #1 Cause of Death

Unknown causes of death are vastly becoming overwhelming in many areas.

In Alberta Canada is has been reported to be the number 1 cause of death for certain individuals in 2022.

The actual death certificates state – Cause of Death: Unknown

The Light of Real Science

We find this unconscionable! We aim to bring the light of real science to the dark world of media and politically controlled science.

All of which is falling apart bit by bit. Every day we hear more bad news as the world crumbles before our eyes across the planet.

The main reason rats are used in experiments is because they are similar to humans in many ways. They share a staggering 90% of genes with humans and their bodily systems function similarly to humans. Additionally, rats can be changed genetically which helps researchers study how certain genes can cause specific diseases in humans. – ScienceABC

Join With Us In This Fight

Please consider joining  with us as a We Talk You Listen’s Buy-a-Rat/Name-a-Rat Series Sponsor.

Each lab rat will be handled and treated within our ethical and moral  standard which we hold all science to. All science needs to be help out for the public to see and understand.

Without opinions or conjecture.

They told us to ”Trust The Science’‘, so we aim to do just that.

However, it will be us conducting the experiments and collecting the data. We also intend to publish the outcomes and finding as soon a possible.

We do all this for the ”Public Good”. Please help us and sponsor a rat!

We rely on all our fanatic support we receive, to bring you the bets of what science can bring.

Thanks you so much for being here with us!

Best Regards.

WTYL Team.


If you would like to join with us and Buy a Rat and be the WTYL Rat Studies Sponsor along with Jana and her rat, Miracle, please click the following link –  https://synapteklabs.com/product/wtyl-rodent-adoption/

WTYL Rodent Adoption “Buy a Rat, Name a Rat”

 

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Global Network Modulation https://synapteklabs.com/global-network-modulation/ https://synapteklabs.com/global-network-modulation/#respond Fri, 19 Aug 2022 22:38:59 +0000 https://synapteklabs.com/?p=3671 Five participants diagnosed with severe medically refractory TS underwent bilateral thalamic DBS stereotactic surgery.

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Global Network Modulation During Thalamic Stimulation for Tourette Syndrome

dulation during thalamic stimulation for Tourette syndrome

Abstract and Figures

Background and objectives Deep brain stimulation (DBS) of the thalamus is a promising therapeutic alternative for treating medically refractory Tourette syndrome (TS).

However, few human studies have examined its mechanism of action. Therefore, the networks that mediate the therapeutic effects of thalamic DBS remain poorly understood.

Methods:

Five participants diagnosed with severe medically refractory TS underwent bilateral thalamic DBS stereotactic surgery.

Intraoperative fMRI characterized the blood oxygen level-dependent (BOLD) response evoked by thalamic DBS and determined whether the therapeutic effectiveness of thalamic DBS, as assessed using the Modified Rush Video Rating Scale test, would correlate with evoked BOLD responses in motor and limbic cortical and subcortical regions.

Results:

Our results reveal that thalamic stimulation in TS participants has wide-ranging effects that impact the frontostriatal, limbic, and motor networks.

Thalamic stimulation induced suppression of motor and insula networks correlated with motor tic reduction, while suppression of frontal and parietal networks correlated with vocal tic reduction. These regions mapped closely to major regions of interest (ROI) identified in a nonhuman primate model of TS.

Conclusions:

Overall, these findings suggest that a critical factor in TS treatment should involve modulation of both frontostriatal and motor networks, rather than be treated as a focal disorder of the brain.

Using the novel combination of DBS-evoked tic reduction and fMRI in human subjects, we provide new insights into the basal ganglia-cerebellar-thalamo-cortical network-level mechanisms that influence the effects of thalamic DBS.

Future translational research should identify whether these network changes are cause or effect of TS symptoms.

Intraoperative-fMRI-during-thalamic-HFS-A-Thalamic-HFS-showing-both-significant


Please open and read PDF here – Global_network_modulation_during_thalamic_stimulation

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Debate on SARS-CoV-2’s https://synapteklabs.com/debate-on-sars-cov-2s/ https://synapteklabs.com/debate-on-sars-cov-2s/#respond Fri, 19 Aug 2022 06:13:58 +0000 https://synapteklabs.com/?p=3466 An Open Debate on SARS-CoV-2’s Proximal Origin is Long Overdue! There is a near consensus view that SARS-CoV-2 has a...

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An Open Debate on SARS-CoV-2’s Proximal Origin is Long Overdue!

SARS-CoV-2

There is a near consensus view that SARS-CoV-2 has a natural zoonotic origin; however, several characteristics of SARS-CoV-2 taken together are not easily explained by a natural zoonotic origin hypothesis.

These include: a low rate of evolution in the early phase of transmission; the lack of evidence of recombination events; a high pre-existing binding to human ACE2; a novel furin cleavage site insert, and high human and mouse peptide mimicry.

Initial assumptions against a laboratory origin, by contrast, have remained unsubstantiated.

Furthermore, over a year after the initial outbreak in Wuhan, there is still no clear evidence of zoonotic transfer from a bat or intermediate species. Given the immense social and economic impact of this pandemic, identifying the true origin of SARS-CoV-2 is fundamental to preventing future outbreaks.

The search for SARS-CoV-2’s origin should include an open and unbiased inquiry into a possible laboratory origin.

Introduction:

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is a novel Betacoronavirus of lineage B (subgenus Sarbecovirus) and the causative agent of Covid-19, the first detected cases of which were identified in Wuhan in December 2019 (Huang et al., 2020).

The near-consensus view of the origin of SARS-CoV-2 is a natural zoonosis (Zhu N. et al., 2020, Wu, F. et al., 2020, Zhou P. et al., 2020).

Bats are thought to be the natural reservoir for SARS-related coronaviruses (SARS-r CoVs) (Li et al., 2005; Wang et al., 2006) and have been identified as the ancestral source from which severe acute respiratory syndrome coronavirus (SARS-CoV) evolved (Janies, 2008; Sheahan, 2008).

Not Been Established

While several intermediate host species have been proposed as the zoonotic source for SARS-CoV-2 (Xiao et al., 2020; Lam et al., 2020; Zhang, T. et al., 2020; Zhou and Shi, 2020), the source of direct bat to human, or intermediate animal to human zoonotic transmission of SARS-CoV-2 has not been established.

An alternative hypothesis, that SARS-CoV-2 leaked from a laboratory, has been widely dismissed (Rasmussen, 2020), yet very few papers counter this theory with data analysis (Andersen et al., 2020; Liu SL. et al., 2020; Graham and Baric, 2020).

Here, we address the main arguments in support of a natural origin of SARS-CoV-2, and outline the various points which support the alternative, that a laboratory origin is still a valid possibility that should not be discounted.

Prevent Future Pandemics

To help prevent future viral pandemics, it is of pivotal importance to identify the source of the virus and this is only possible with an unbiased analysis of all data available.

We couple this work with calls from recent opinion pieces and comparative studies questioning a zoonotic origin (Sousa, 2020; Sirotkin & Sirotkin 2020; Relman, 2020; Segreto & Deigin 2020; Butler, 2020; Sallard et al., 2021) via a review of the latest literature, and propose an alternative to the natural zoonosis hypothesis.


Please visit the ResearchGate paper below –

https://www.researchgate.net/publication/349125078_An_open_debate_on_SARS-CoV-2’s_proximal_origin_is_long_overdue

 

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